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Leaky expression of PRMT1 in Escherichia coli and redox regulation of its histone methyltransferase activity

Kim et al. | Oct 07, 2026

Leaky expression of PRMT1 in <i>Escherichia coli</i> and redox regulation of its histone methyltransferase activity

This study characterizes recombinant human Protein arginine methyltransferase 1 (PRMT1) expressed in Escherichia. coli and reveals that the enzyme exhibits leaky expression even without isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. Through in vitro histone methyltransferase assays, we demonstrate that PRMT1 activity toward histone H4R3 is enhanced by dithiothreitol (DTT) in a dose-dependent manner but remains unresponsive to β-mercaptoethanol. These findings highlight the critical role of redox conditions in regulating PRMT1 catalytic function and provide a practical framework for future biochemical and kinetic studies.

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Optimizing AI image detection using a Convolutional Neural Network model with Fast Fourier Transform

Gupta et al. | Oct 24, 2025

Optimizing AI image detection using a Convolutional Neural Network model with Fast Fourier Transform

Recent advances in generative AI have made it increasingly hard to distinguish real images from AI-generated ones. Traditional detection models using CNNs or U-net architectures lack precision because they overlook key spatial and frequency domain details. This study introduced a hybrid model combining Convolutional Neural Networks (CNN) with Fast Fourier Transform (FFT) to better capture subtle edge and texture patterns.

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Exploring the Factors that Drive Coffee Ratings

Agarwal et al. | May 19, 2025

Exploring the Factors that Drive Coffee Ratings

This study explores the factors that influence coffee quality ratings using data from the Coffee Quality Institute. Through a regression model based on gradient descent, the authors aimed to predict coffee ratings (total cup points) and hypothesized that sweetness and the coffee producer would be the most influential factors.

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The optimization of high-protein duckweed cultivation in eutrophicated water with mutualistic bacteria

Akkarajeerawat et al. | Mar 18, 2025

The optimization of high-protein duckweed cultivation in eutrophicated water with mutualistic bacteria

he rapid growth of the human population is driving food crises in Thailand and Southeast Asia, while contributing to global food insecurity and a larger carbon footprint. One potential solution is cultivating duckweed (Wolffia globosa) for consumption, as it grows quickly and can provide an alternative protein source. This research explored two methods to optimize duckweed cultivation: using phosphorus- and nitrogen-rich growing media and plant growth-promoting bacteria (PGPB).

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